A cikin samar da manyan flanges, akwai dalilai da yawa da suka shafi ayyukan manyan flanges. Da ke ƙasa muna cewa yawancin abubuwan da aka gama gari, na farko shine yanayin zafin jiki (zafin jiki shine lokacin da kashi 50% na firam ɗin sune zafin jiki wanda aka bayyana zazzabi a matsayin ninki biyu. Babban kwayar halittar DNA babban mahimmanci ne da ke shafar tsarin PCR din. A karkashin yanayi mai kyau ya isa yadda ake so. Zazzabi ya fito ne daga 55 ° C to 70 ° C. zazzabi mai zafin jiki an saita shi 5 ° C ƙasa da TM na farkon.

Ko an kai yawan zafin jiki. Zazzabi mai zafin jiki ya kai yawan zafin da ake buƙata. Babban flakeci na sikelin-sikelin flake ne gaba daya ta hanyar ingantaccen maganin zafi mai zafi, wanda ake kira "annealing", daidaitaccen zafin jiki). Hakanan zaka iya kiyaye ramin kallo na wutar tanderace. Babban flanige ya dace a cikin wani yanki na zamani ya kamata ya kasance a cikin jihar Incarescent, amma babu mai laushi da sagging.

Bi ta sealing na jikin wutar terne (waje harsashi yana welded tare da farantin karfe da faranti, trolley ya wuce taushi mai laushi da yashi na sepple da kuma sakin yashi don rage zafin rana Kuma hasara mai hasara, kuma tabbatar da sealing na jikin wutar terence.), ya kamata a rufe wutar lantarki mai haske daga iska ta waje; Ta amfani da hydrogen a matsayin mai kare mai kare, tashar jiragen ruwa guda ɗaya kawai tana buɗe (ana amfani da ita don kunna wutar hydrogen). Ana iya amfani da hanyar dubawa zuwa ga gibin gidajen abinci na wutar lantarki da ruwan soapy don ganin idan gas ke gudana. Mafi sauƙi wurin da za a yi gas shine wurin da wutar tukunan kebul ɗin ke shiga bututu da wurin ana fitar da bututun. Zoben hatimin a wannan wurin yana da yiwuwa a sa. Koyaushe duba akai-akai don canzawa.

Abu na biyu, matsakaicin gas mai kariya, don hana abin da ya faru na micro-Leak, gas mai ƙarfi ya kamata ya kula da wani matsin lamba na kariya, idan yana buƙatar fiye da 20kbar. Ana amfani da yanayin yanayi: gabaɗaya, ana amfani da tsabta hydrogen azaman yanayin yanayi, da kuma tsarkakakkiyar yanayin shine zai fi dacewa 99,99% ko fiye. Idan wani bangare na sararin samaniya shine iskar gas, tsarkakakku na iya zama ƙasa, amma dole ne bai ƙunshi oxygen ko tururi mai yawa ba.