Da farko, kafin zabar rawar rawar soja, bari mu dubi menene matsalolin machining da bakin karfe flange? Nemo da wuya maki iya zama sosai m, da sauri don nemo amfani da rawar soja. Menenematsaloli na bakin karfe flange aiki?Brief sanda wuka: bakin karfe aiki guntu karfi da kuma m, yankan zafin jiki ne sosai high.Lokacin da karfi da kuma m guntu kwarara ta gaban surface na wuka, zai kai farmaki bonding, Fusion waldi da sauran bonding sabon abu, shafi aiki sassa na Hasken saman; Taurin aikin yana da tsanani.
A cikin amfani da manyan flanges, akwai abubuwa masu aiki da yawa da ke shafar manyan flanges. Wadannan abubuwa ne da yawa gama gari. Na farko shi ne zafin jiki mai raɗaɗi (zazzabi mai raɗaɗi shine ma'aunin zafin jiki na firamare da samfuri bi da bi, da zafin jiki lokacin da kashi 50% na firamare da ƙarin jeri ake wakilta azaman ƙwayoyin DNA masu ɗaure biyu).

Yana da mahimmancin mahimmanci wanda ke shafar ƙayyadaddun PCR.A cikin yanayin sha'awar, zafin jiki na annealing yana da ƙananan isa don tabbatar da cewa ƙaddamarwa da jerin abubuwan da ake nufi suna da amfani kuma suna da girma don rage ƙayyadaddun rabuwa. jeri daga 55 ℃ zuwa 70 ℃. The annealing zafin jiki ne kullum saita 5 ℃ kasa fiye da Tm na primers.

Daga: 168 jabun net