Ekwenziweni kwama-flanges amakhulu, kunezinto eziningi ezithinta ukusebenza kwamafashi amakhulu. Ngezansi sisho izinto eziningana ezijwayelekile, okokuqala yindawo yokushisa eyengezwayo (izinga lokushisa le-annelling yipharamitha yokushisa lapho i-primer nethempulethi zihlanganiswa, lapho ama-50% ama-primers athathwa khona ngokulandelana okuphindwe kabili I-DNA Molecule eqinile iyinto ebalulekile ethinta ukucaciswa kwe-PCR. Ngaphansi kwezimo ezifanele, amazinga okushisa anvelling aphansi ngokwanele ukuqinisekisa ukuthi ama-primers ancishiswe ngokulandelana okufiselekayo, ngenkathi ephakeme ngokwanele ukunciphisa ukubopha okungacaciswanga. Ukushisa kusuka ku-55 ° C kuya ku-70 ° C. Ukushisa okushisa okuvame ukusetha ama-5 ° C aphansi kune-TM ye-Primer.

Ukuthi izinga lokushisa elicacisiwe lifinyelelwe. Izinga lokushisa elivelelayo lifinyelela izinga lokushisa elidingekayo. Ukwelashwa okungaphezulu kwe-Flange kuvame ukwenziwa ngokwelashwa okuqinile kwesixazululo, okuvame ukubizwa ngokuthi "ukuhlanganisa", futhi uhla lokushisa lungu-1040 ~ 1120 ° C (I-Japan Standard). Ungase futhi ubheke emgodini wokubheka wesithando se-annelling. Ama-fittings amakhulu we-flange endaweni yokunyakazisa kufanele abe sesimweni se-incandescent, kepha akukho okuthambisayo nokugcotshwa.

Kulandelwa ukubekwa uphawu kwesidumbu somlilo (i-auter igobolondo ifakwe ngeplate yensimbi nensimbi yensimbi, i-trolley ifakwe ngensimbi yensimbi nensimbi, i-trolley idlula oxhumana naye othambile nendlela yokufaka uphawu yesithako ukuze unciphise imisebe yokushisa kanye nokulahleka kwe-convection, futhi ngempumelelo ukuqinisekisa ukubekwa uphawu kwesidumbu somlilo.), kufanele kuvalwe isithando somlilo esikhanyayo futhi sihlukaniswe nomoya ongaphandle; Usebenzisa i-hydrogen njengogesi wokuvikela, itheku elilodwa kuphela eliphumayo elivulekile (elisetshenziselwa ukusula i-hydrogen ekhishwe). Indlela yokuhlola ingafakwa ezikhampani zamalunga wesithando somlilo esivelelayo ngamanzi anensipho ukuze ubone ukuthi igesi isebenza yini. Indawo elula kakhulu yokuqalisa igesi yindawo lapho isithando se-Annealing singena khona ipayipi nendawo lapho ipayipi likhishwa khona. Indandatho yokubopha uphawu kule ndawo ithambekele ekugqokeni. Hlala njalo uhlole njalo ukuze ushintshe.

Okwesibili, ingcindezi yegesi evikelayo, ukuze ivikele ukwenzeka kokuvuza okuncane, igesi yokuzivikela kufanele ilondoloze ingcindezi ethile enhle, uma igesi evikela i-hydrogen, ngokuvamile idinga ngaphezu kwe-20Kbar. I-Anneling Atmosphere: Ngokuvamile, i-hydrogen emsulwa isetshenziswa njengomoya ongenisa umkhathi, futhi ubumsulwa bomkhathi kungcono 99.99% noma ngaphezulu. Uma enye ingxenye yomkhathi ingugesi we-inert, ubumsulwa bungaphansi, kepha akufanele libe ne-oksijini eningi noma amanzi.