Okokuqala, ngaphambi kokukhetha i-drill bit, bheka ubunzima be-stainlessinsimbi flangeukucubungula. Thola ubunzima kungaba olunembile kakhulu, ngokushesha kakhulu ukuthola ukusetshenziswa sokuprakthiza. Yibuphi ubunzima ekucutshungulweni kwe-flange yensimbi engagqwali? Ummese onamathelayo: ukuqina kwe-chip yensimbi engagqwali, izinga lokushisa lokusika liphezulu kakhulu. Lapho i-chip eqinile igeleza endaweni yokusika yangaphambili, izokwenzeka ukunamathela, i-fusion welding kanye nezinye izinto zokunamathela, ezithinta ukugqama kokubukeka kwezingxenye ezicutshunguliwe; Ukuqina komsebenzi kunzima.

Ekusetshenzisweni kweama-flange amakhulu, kunezici eziningi zokusebenza ezithintaama-flange amakhulu. Lapha sikhuluma ngezinto ezimbalwa ezivamile. Esokuqala izinga lokushisa le-annealing (izinga lokushisa le-annealing yipharamitha yezinga lokushisa le-primer nesifanekiso ngokulandelana kwazo, lapho u-50% we-primer nokulandelana okuhambisanayo kuyizinga lokushisa lama-molecule e-DNA anemicu ekabili.

Kuyisici esibalulekile esithinta ukucaciswa kwe-PCR. Endabeni ye-aspiration, izinga lokushisa le-anneal liphansi ngokwanele ukuze kuqinisekiswe ukuthi i-primer iphazanyiswa ngokulandelana okuqondiwe, futhi iphakeme ngokwanele ukunciphisa ukuhlukana okungaqondile. Amazinga okushisa e-anneal anengqondo asukela ku-55℃ kuya ku-70℃. Izinga lokushisa le-anneal ngokuvamile libekwe ku-5℃ ngaphansi kwe-Tm ye-primer.