Okokuqala, ngaphambi kokukhethwa kwe-drill, bheka i-stainlessinsimbi flangeukucubungula kunzima kuyini? Thola ukuthi ubunzima bungaba nembe kakhulu, ngokushesha kakhulu ukuthola ukusetshenziswa kwe-drill bit. Buphi ubunzima phakathiinsimbi engagqwali flangeiyacubungula? Ummese wenduku emfushane: I-stainless steel processing chip iqinile futhi iqinile, izinga lokushisa lokusika liphezulu kakhulu. Lapho i-chip eqinile igeleza ebusweni bommese wangaphambili, izohlasela i-bonding, i-fusion welding nokunye okunamathelayo, kuthinte ukukhanya okungaphezulu kwezingxenye zokucubungula; Ukuqina komsebenzi kunzima.
Ekusetshenzisweni kweama-flange amakhulu, kunezici eziningi ezithinta umsebenzi weama-flange amakhulu, okulandelayo sithi izici ezimbalwa ezivamile, eyokuqala yizinga lokushisa le-annealing (izinga lokushisa le-annealing yipharamitha yokushisa ye-primer kanye nesifanekiso ngokulandelana, lapho amaphesenti angu-50 we-primer nokulandelana okuhambisanayo izinga lokushisa lama-molecule e-DNA anemicu ephindwe kabili.
Kuyisici esibaluleke kakhulu esithinta I-SPECIFICITY ye-PCR. Esimeni sesifiso sokuvelela, izinga lokushisa le-anneal liphansi ngokwanele ukuqinisekisa ukuthi i-primer iwusizo ekucimeni ngokulandelana okuqondiwe, futhi iphakeme ngokwanele ndawonye ukuze kuncishiswe ukungacacisi, ngokulandelanayo. Izinga lokushisa elizwakalayo lokudonsa lisuka ku-55 kuye ku-70 livamise ukuthi libe ngaphansi kuka-5 kune-Tm ye-primer.
Isikhathi sokuthumela: Oct-13-2020