Kwimveliso yeflene enkulu, zininzi izinto ezichaphazela intsebenzo yeflengu enkulu. Apha ngezantsi sithetha izinto ezininzi eziqhelekileyo, iqondo lokuqala libubushushu (amaqondo obushushu yiphamela yobushushu xa i-primer idityanisiwe, xa i-template idityanisiwe, I-DNA Molecule ye-DNA ibaluleke kakhulu echaphazela ubuchwephesha be-PCR. Phantsi kweemeko ezifanelekileyo, amaqondo obushushu aphantsi athontelwa ngokwaneleyo ukuqinisekisa ukuba i-Primes ilandelelana. I-Anneing eNgcono Iqondo lokushisa livela kwi-55 ° C ukuya kuma-70 ° C. Iqondo lobushushu lisetwa i-5 ° C ezisezantsi kune-rimmer.

Ingaba iqondo lobushushu lifikile. Amaqondo obushushu afikelela kubushushu obufunekayo. Unyango olukhulu lwe-flanges luqhutywa ngonyango oluqinileyo lwesisombululo, olubizwa ngokuba 'lubizwa ngokuba "lulonwa", kwaye uluhlu lwamaqondo obushushu yi-1040 ~ ° C (isiJapan). Unokujonga nomngxunya wokujonga izitovu. I-flat enkulu yeflethi kwindawo yokwakhela kufuneka ibe kwimeko ye-incandases, kodwa akukho nto ityhilayo kunye nokungcungcutheka.

Ilandelwa kukutywina komzimba wesithando somlilo (iqokobhe langaphandle lineplate yensimbi kunye ne-trolley i-welday, itroli yentsimbi, itroli yentsimbi, indawo yokutywina yesanti yokunciphisa umthamo wobushushu kunye nokulahleka okugqithisayo, kunye nokuqinisekisa ukutyhileka komzimba wesithando somlilo.), Izithando zomlilo eziqaqambileyo kufuneka zivaliwe kwaye zihlukane nomoya ongaphandle; Sebenzisa i-hydrogen njengegesi yokukhusela, inye kuphela i-port evulekileyo (esetyenziselwa ukubeka i-hydrogen ekhutshiweyo). Indlela yokuhlolwa inokusetyenziswa kwizithuba zokudibana kweziko elihlekisayo kunye namanzi anesepha ukuze ubone ukuba irhasi iyasebenza. Eyona ndawo ilula yokuqhuba igesi yindawo apho isiko leziyolo elihlekisayo lingena khona umbhobho kunye nendawo apho kuthunyelwe khona umbhobho. Iringi yokutywina kule ndawo ixhotyiswe kakhulu ukuba inxibe. Soloko ujonge rhoqo ukutshintsha.

Okwesibini, uxinzelelo lwegesi olukhuselekileyo, ukwenzela ukuthintela ukwenzeka kokwenzeka okuncinci, igesi yokukhusela iziko kufuneka igcine uxinzelelo oluthile, ukuba luyi-hydrogen irhasi, ngokubanzi ifuna ngaphezulu kwe-20kbar. Imo yezulu ephilayo: Ngokubanzi, i-hydrogen emsulwa isetyenziswa njengemozulu ephilayo, kunye nokucoceka kwemozulu kukhetho lwe-99.99% okanye ngaphezulu. Ukuba enye inxenye yemozulu yirhasi ye-interter, ubunyulu bunokuba phantsi, kodwa akufuneki bube neoksijini enkulu okanye i-vapor yamanzi.