Okokuqala, ngaphambi kokukhetha i-drill bit, jonge kubunzima kwi-stainightI-Flange yentsimbiukuqhubekeka. Fumanisa ubunzima kunokuba luchaneke kakhulu, ngokukhawuleza kakhulu ukufumana ukusetyenziswa kwe-drill. Bubuphi ubunzima kwizinto ezingekhoyo ngentsimbi yentsimbi? I-theinking phumela: Intsimbi engenasinqwazi ye-chip ye-chip, amaqondo obushushu anqabileyo aphezulu kakhulu. Xa i-chip eyomeleleyo iqala kwindawo yokusika ngaphambili, iya kubakho, i-wlion yeldion kunye nenye into enamathela eyokuncamathela, echaphazela inkangeleko yamalungu agqityiweyo; Umsebenzi oqaqambileyo kakhulu.

Ekusebenziseni kweIiflethi ezinkulu, zininzi izinto ezichaphazelayo ezichaphazelaIiflethi ezinkulu. Apha sithetha ngezinto ezininzi eziqhelekileyo. Eyokuqala bubushushu obuthandwayo (ubushushu obukhaliyo yiphamela yobushushu be-primer kunye netemplate ngokulandelelana, xa i-50% ye-primer kunye nokulandelelana kweqondo le-DNA kwiimolekyuli zeDNA eziphithizelayo.

Yinto ebalulekileyo echaphazela ubuso be-PCR. Kwimeko yokunqwenela, amaqondo obushushu athandekayo aphantsi ngokwaneleyo ukuqinisekisa ukuba i-primer ichithwe kunye nokulandelelana, nangaphezulu ngokwaneleyo ukunciphisa ukwahlula-hlula. Amaqondo obushushu afanelekileyo avela ku-55 ℃ ukuya kuma-70 ℃. Iqondo lobushushu elibonakalayo lisetwa kwi-5 ℃ iphantsi kune-primer.