Okokuqala, ngaphambi kokuba ukhethe i-drill bit, jonga ubunzima kwi-stainlessintsimbi yentsimbiukuqhubekeka. Fumana ubunzima bunokuchaneka kakhulu, ngokukhawuleza kakhulu ukufumana ukusetyenziswa kwe-drill. Bubuphi ubunzima ekusetyenzweni kweflange yentsimbi engenastainless? Imela encangathi: Ukuqina kwetshiphu yentsimbi engatyiwayo, ubushushu bokusika buphezulu kakhulu. Xa i-chip eyomeleleyo ihamba nge-front cutter surface, iya kwenzeka i-adhesive, i-fusion welding kunye nezinye i-adhesive phenomenon, echaphazela ukubonakala kokukhanya kweendawo ezicutshungulwayo; Ukuqina komsebenzi kunzima.

Ekusetyenzisweni kweiiflange ezinkulu, kukho izinto ezininzi ezisebenzayo ezichaphazelayoiiflange ezinkulu. Apha sithetha ngezinto ezininzi eziqhelekileyo. Eyokuqala yi-annealing temperature (iqondo lobushushu le-annealing yiparameter yobushushu be-primer kunye netemplate ngokulandelelanayo, xa i-50% ye-primer kunye nolandelelwano oluhambelanayo bubushushu bee-molecule ze-DNA eziphindwe kabini.

Yinto ebalulekileyo echaphazela ubunjani bePCR. Kwimeko yomnqweno, iqondo lokushisa le-annealing liphantsi ngokwaneleyo ukuqinisekisa ukuba i-primer ihlanjululwe ngokulandelelana okujoliswe kuyo, kwaye iphezulu ngokwaneleyo ukunciphisa ukwahlukana okungachazwanga. Amaqondo obushushu afanelekileyo aqala kwi-55℃ ukuya kuma-70℃. Ubushushu be-annealing ngokuqhelekileyo busetwa ku-5℃ ngaphantsi kwe-Tm ye-primer.