Okokuqala, ngaphambi kokuba ukhethe i-drill, jonga i-stainlessintsimbi yentsimbiprocessing nzima yintoni? Fumana ubunzima bunokuchaneka kakhulu, ngokukhawuleza ukufumana ukusetyenziswa kwe-drill bit. Ziziphi iingxakiintsimbi engenasiciukuqhubekeka? Imela emfutshane yentonga: Itshiphu yokucoca intsimbi eyomeleleyo kwaye yomelele, ubushushu bokusika buphezulu kakhulu. Xa i-chip eyomeleleyo ihamba kumphezulu wemela yangaphambili, iya kuhlasela i-bonding, i-fusion welding kunye nezinye izinto ezincamathelayo, zichaphazela ukuqaqamba komphezulu weendawo zokucubungula; Ukuqina komsebenzi kunzima.
Ekusetyenzisweni kweiiflange ezinkulu, kukho izinto ezininzi ezichaphazela umsebenzi weiiflange ezinkulu, oku kulandelayo sithetha izinto ezimbalwa eziqhelekileyo, eyokuqala iqondo lobushushu le-annealing (iqondo lobushushu le-annealing yiparameter yobushushu be-primer kunye netemplate ngokulandelelanayo, xa i-50 pesenti ye-primer kunye nolandelelwano oluhambelanayo bubushushu bee-molecule ze-DNA eziphindwe kabini.
Yinto ebaluleke ngakumbi echaphazela INKCAZELO ye-PCR. Kwimeko yebhongo, iqondo lokushisa le-annealing liphantsi ngokwaneleyo ukuze kuqinisekiswe ukuba i-primer iluncedo ekucimeni ngokulandelelana okujoliswe kuyo, kwaye iphezulu ngokwaneleyo kunye nokunciphisa ukungacaciswanga, ngokulandelanayo. Ubushushu obufanelekileyo bokucoca ukusuka kwi-55 ukuya kwi-70 ngokuqhelekileyo lusetelwe ukuba lube ngu-5 ngaphantsi kwe-Tm ye-primer.
Ixesha lokuposa: Oct-13-2020