Mukugadzirwa kwemaflange makuru, pane zvinhu zvakawanda zvinokanganisa kushanda kwemaflange makuru. Pazasi tinoti zvinhu zvakati wandei zvakajairika, yekutanga ndiyo tembiricha yekudziya (iyo tembiricha yekudziya ndiyo tembiricha parameter kana primer uye template zvabatanidzwa, kana 50% yemaprimers ari Tembiricha painoteedzana inoteedzana inotaridzwa kaviri- stranded DNA molecule chinhu chakakosha chinokanganisa kujeka kwePCR Pasi pemamiriro ezvinhu akanaka, tembiricha yeannealing yakadzikira zvakakwana kuti ive nechokwadi chekuti maprimers anonamirwa kutevedzana kwaidiwa, uku ichikwira zvakakwana kuti ideredze Kusina-chaiwo kusungirirwa tembiricha iri kubva 55 ° C kusvika 70 ° C. Annealing tembiricha inowanzosetwa 5 ° C yakaderera pane Tm yekutanga.

Kana tembiricha yakatarwa yasvika. Annealing tembiricha inosvika tembiricha inodiwa. Huru-mwero flange kurapwa kunowanzoitwa neyakaoma mhinduro kupisa kurapwa, iyo inowanzonzi "annealing", uye tembiricha yemhando i1040 ~ 1120 °C (yeJapan standard). Iwe unogona zvakare kutarisa kuburikidza negomba rekutarisa rechoto chinopisa. Iyo yakakura flange fittings munzvimbo yeannealing inofanirwa kunge iri incandescent state, asi hapana kupfava uye kudonha.

Inoteverwa nekuiswa chisimbiso chemuviri wechoto (yekunze goko rakasungwa nesimbi ndiro uye chikamu simbi, trolley inosungwa nesimbi uye simbi ndiro, trolley inopfuudza yakapfava yekubata nechoto chebira uye jecha rekuvhara nzira yekudzikisa kupisa kwemwaranzi. uye kurasikirwa kweconvection, uye nekunyatso simbisa kuiswa chisimbiso chevira revira ), iyo inopenya yevira yevira inofanira kuvharwa uye yakaparadzaniswa nemhepo yekunze; uchishandisa haidrojeni segasi rekudzivirira, chiteshi chimwe chete chekuburitsa chinovhurwa (chinoshandiswa kudzima haidhirojeni yaburitswa). Nzira yekuongorora inogona kushandiswa kune mazamu emajoini echoto chinopisa nemvura ine sipo kuona kana gasi riri kushanda. Nzvimbo iri nyore kwazvo yekumhanyisa gasi inzvimbo iyo choto chinopisa chinopinda mupombi uye nenzvimbo inoburitswa pombi. Mhete yekuisa chisimbiso munzvimbo ino inonyanya kupfekwa. Gara uchiongorora kuti uchinje.

Chechipiri, iyo inodzivirira gasi kudzvanywa, kuitira kudzivirira kuitika kweiyo micro-leakage, iyo choto inodzivirira gasi inofanirwa kuchengetedza imwe yakanaka kudzvanywa, kana iri hydrogen inodzivirira gasi, kazhinji inoda kupfuura 20kBar. Anealing atmosphere: Kazhinji, haidrojeni yakachena inoshandiswa semhepo inodziya, uye kuchena kwemhepo kuri nani 99.99% kana kupfuura. Kana chimwe chikamu chemumhepo chiri gasi inert, kuchena kunogona kuve kwakaderera, asi hakufanirwe kuve neokisijeni yakawandisa kana mhute yemvura.